Dry of Microencapsulated Lactic Acid Bacteria-effects of Trehalose and Immobilizationon Cell Survival and Release Properties
(0) Lactic acid bacteria(LAB)were capsulated with alginate,gelatin and trehalose additive by extrusion method and dried at 4℃.The microcapsules were generally spherical and had a wrinkled surface with a size of 1.7±0.2mm.Trehalose as carbohydrate source in culture medium could reduce acid produce and had no function on positive proliferation of LAB.Using trehaiose as carbohydrate source and protective medium simultaneously had a benefit in protection LAB cells during the storage at 4℃,and the live LAB cells could be at l07 CFU/g after storage 8 weeks.Cells of LAB could be continuously released from the capsules from the acidic condition(pH 1.2)to the neutral condition(pH 6.8),and the release amounts and speeds of LAB cells in neutral medium were much higher and faster than that in acidic condition.Additionally,immobilization of LAB could improve the survival of cells when they were exposed to acidic medium(pH 1.2)by the survival ratio of 76%.
Alginate Gelatin Micracapsule Survival Release Tolerance
Li Xiao-yan Chen Xi-guang
College of Marine Life Science,Ocean University of China,Qingdao 266003,China
国内会议
青岛
英文
192-200
2007-08-01（万方平台首次上网日期，不代表论文的发表时间）