Diagnostic serum proteomic analysis in patients with active tuberculosis
Background:The diagnosis for smear-negative pulmonary tuberculosis (TB) is very difficult.Proteomic finger-printing of sera is a potentially useful tool.Methods:This study analyzed the results of the proteomic fingerprinting in sera obtained from active TB patients and controls using the surface-enhanced laser desorption ionization time of flight mass spectrometry (SELD1-TOF-MS) and protein-chip technology.The peaks were detected and analyzed,and a diagnostic system was developed.The protein peak was identified using high performance liquid chromatography (HPLC)-tandem matrix-assisted laser desorption/ionization-TOF-MS (MALDI-TOF-MS).Results:Around 50 protein peaks were found significantly different between the TB patients and the controls (P<0.01).Three protein peaks at m/z 5643,4486 and 4360 were selected for system classification and the development of a decision model.The model distinguished the TB patients from the controls with a sensitivity of 96.9% and a specificity of 97.8%,respectively.The diagnostic accuracy was up to 97.3%.The one most discrepant protein peak at m/z 5643 seen in sera of active TB patients was identified as orosomucoid.Conclusions:A diagnostic system for active TB was developed using mass spectrometry and protein chip technology and required only small-volume serum samples.One potential protein biomarker at m/z 5643 was identified as orosomucoid.
Pulmonary tuberculosis Diagnosis Proteomics Mass spectrometry
Junxian Zhang Xueqiong Wu Lilan Shi Yan Liang Zhensheng Xie Yourong Yang Zhongxing Li Cuihua Liu Fuchuan Yang
Army Tuberculosis Key Laboratory,Institute of Tuberculosis Research,the 309th Hospital of PLA,Beijin Proteomic Platform,Institute of Biophysics,Chinese Academy of Science,Beijing 100101,China Beijing DeYi Biological ε Medical Technology Co.Ltd,Beijing 100035,China
国内会议
武夷山
英文
123-127
2012-12-22（万方平台首次上网日期，不代表论文的发表时间）