Elevated expression of Tim-3 on CD8 T cells correlates with disease severity of pulmonary tuberculosis
Objectives:T cell immunoglobulin and mucin domain-containing molecule 3 (Tim-3) plays an important role in regulating T cell responses and induction of peripheral tolerance.In this study,the association of Tim-3 with active pulmonary tuberculosis (TB) was investigated.Methods:Tim-3 expression on CD8 T cells and its influence on production of effector molecules and proliferation of CD8 T cells were examined.Results:Pulmonary TB patients had significantly elevated Tim-3 expression on total CD8 T cells (P < 0.0001) and on antigen-specific CD8 T cells (P =0.0028) compared with tuberculin-positive healthy controls.The expression of Tim-3 on CD8 T cells was significantly higher in smear/ culture-positive TB patients than smear/culture-negative TB patients (P < 0.0001).Tim-3 expressing CD8 Tcells had reduced production of IFN-γ,decreased degranulation and lower proliferation in response to Mycobacterium tuberculosis antigen stimulation.Blocking of Tim-3 signaling led to significantly increased production of IFN-γ.Conclusions:The elevated expression of Tim-3 on CD8 T cells correlates with functional defects of CD8 T cells and disease severity of pulmonary TB.
Tuberculosis Tim-3 CD8 T cells Functional defects
xinjing wang Zhihong Cao Jing Jiang Yanfeng Li Mei Dong Mario Ostrowski Xiaoxing Cheng
Division of Research,Institute of Tuberculosis,309 Hospital,17 Hei Shan Hu Road,Haidian,Beijing 1000 First Department of Tuberculosis,Institute of Tuberculosis,309 Hospital,Beijing,China Department of Laboratory Medicine,309 Hospital,Beijing,China Clinical Sciences Division,University of Toronto,Medical Sciences Building,1 King”s College Circle,T
国内会议
武夷山
英文
145-154
2012-12-22（万方平台首次上网日期，不代表论文的发表时间）