Identification of a novel IRGM promoter single nucleotide polymorphism associated with tuberculosis
Background:Human immunity-related GTPase M (IRGM) is found to play an important role in defense against intracellular pathogen Mycobacteriurn tuberculosis in vitro by regulating autophagy.To verify whether single nucleotide polymorphisms (SNPs) in the promoter region of IRGM gene are assodated with tuberculosis (TB) 1.7 kb IRGM promoter region was sequenced and SNP analysis was conducted in TB patients and healthy controls.Methods:A simple and rapid procedure for extracting DNA from clotted-blood was developed in this study.A 1.7 kb IRGM promoter region was amplified and sequenced for nucleotide polymorphism search.Then,3 SNPs were selected and analyzed in 216 TB patients and 275 healthy subjects by ligase detection reaction technique.Results:DNA extracted by our method was of high quality and suitable for PCR,sequencing,and genotyping.We identified 29 polymorphisms in the 1.7 kb IRGM promoter region,including 11 novel polymorphisms not yet reported.Large population analysis showed that frequencies of-1208A allele (P =0.031),-1208AA genotype (P =0.042),and-1208A/-1161C/-947C (P =0.035) and-1208G/-1161C/-947C (P =0.030) haplotypes in cases were significantly different from those in controls.Conclusions:In 1.7 kb IRGM promoter region,only-1208A/G polymorphism is associated with susceptibility to TB.
IRGM gene Susceptibility Tuberculosis DNA extraction Clotted blood
Nanying Che jianyuan Zhang Weicong Ren Miao Tian Yan Li Wensheng Li Jun Cheng Chuanyou Li Song Li Tiejie Gao Zhiguo Zhang Yuefei Han Xuxia Zhang Yong Sun Yi Liu Zhaogang Sun
Department of Bacteriology and Immunology,Beijing Tuberculosis ε Thoracic Tumor Research Institute.T Department of Bacteriology and Immunology,Beijing Tuberculosis ε Thoracic Tumor Research Institute.T Beijing Changping Center for Tuberculosis Control and Prevention,Changping District,Beijing,102200,P
国内会议
武夷山
英文
171-176
2012-12-22（万方平台首次上网日期，不代表论文的发表时间）