Effects of the extract from Yangjing capsule, a traditional Chinese medicine, on steroidogenesis and apoptosis in mouse Leydig tumor cells
In this study,we investigated the effects of the Yangjing capsule extract,which is used to treat male sexual dysfunction and infertility,on steroidogenesis and apoptosis in testicular Leydig cells.Mouse Leydig tumor cells (MLTC-1) were cultured and treated with Yangjing capsule extract (0.01-1 mg/mL) for 24 h.The testosterone levels in the medium were evaluated by radioimmunoassay.Expression of steroidogenic key enzymes (StAR,CYP11A1,HSD3B) was examined using real-time RT-PCR and immunoblotting.Subsequently,MLTC-1 were treated with Yangjing capsule extract (0.01-1 mg/mL) for 48 h in a serum-free medium.Cell viability was measured by MTT assays.The cell cycle and apoptosis were analyzed using flow cytometry.The expression of activated caspase-3 was analyzed using RT-PCR and a colorimetric protease assay.Yangjing capsule extract showed higher testosterone production and the expression of StAR,CYP11A1,and HSD3B mRNAs and proteins than those in control group in MLTC-1.In addition,H89,a PKA inhibitor,significantly inhibited Yangjing-stimulated testosterone production.In another experiment,Yangjing capsule extract improved the cell viability of MLTC-1 and decreased the number of cells in the G0/G1 phase while increasing the number of cells in the S-phase in a dose-dependent manner.Further investigation showed that Yangjing capsule extract prevented cell apoptosis by inhibiting the expression of caspase-3.These results showed that Yangjing capsule extract can improve steroidogenesis and inhibit apoptosis in MLTC-1.
Yangjing capsule testosterone steroidogenesis apoptosis caspase-3
Dalin Sun Yugui Cui Baofang Jin Xindong Zhang Xiaoyu Yang Chao Gao
Institute of Andrology, Nanjing University of Chinese Medicine, PR China State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affil Institute of Andrology, Nanjing University of Chinese Medicine, PR China ; State Key Laboratory of R
国内会议
江苏南通
英文
355-368
2012-10-01(万方平台首次上网日期,不代表论文的发表时间)