RP-HPLC analysis of diosgenin in Rhizoma Dioscoreae Nipponicae
Objective:To establish an RP-HPLC method for determination of diosgenin in Rhizoma Dioscoreae Nipponicae,giving the reference for controlling the intrinsic quality.Methods:Firsdy,Diosgenin was extracted by two-phase acid hydrolysis from dry herbal material.Secondly,sample solutions were analyzed on Agilent Zorbax Extend C18 column (4.6 min×250 mm,5 μm)at 40℃.The diosgenin was separated with acetonitrile-pure water (94∶6) as a mobile phase at a flow rote of 1.0 mL·min-1 under a ultraviolet detection wavelength of 203 nm.Results:Diosgenin could be separated obviously from the interferential components in sample solutions.The linearity of the calibration cure (r=0.9999) was good within the range of 1.055-21.10 μg.RSD of repeated experiments (n=6) was 1.7% with average recovery (n=6) reached 96.8% (RSD=1.5%).The average content (n=6) of diosgenin in Rhizoma Dioscoreae Nipponicae was 1.61% (RSD=1.7%).Conclusions:This method can sensitively make a quantitation of diosgenin with a simple pretreatment,and has a broad linear range and good repeatability,which makes it suitable for determination of diosgenin in Rhizoma Dioscoreae Nipponicae.
Rhizoma Dioscoreae Nipponicae Diosgenin Two-phase acid hydrolysis RP-HPLC
YANG Huan XIAO Xiao-juan SHA Juan SHEN Yu-ping YANG Ke-di CHEN Jun
School of Pharmaceutical Science,Nanjing University of Traditional Chinese Medicine,Nanjing 210029,C School of Pharmaceutical Science,Jiangsu University,Zhenjiang 212001,China School of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004,China
国内会议
苏州
英文
663-670
2006-11-04（万方平台首次上网日期，不代表论文的发表时间）