Isolation and Molecular Characterization of Toxoplasma gondii from Chickens in China
One strain of Toxoplasma gondii was successfully isolated from chickens in China by bioassay in mice.Antibodies and circulating antigens of T.gondii were assayed by the ELISA kits in 100 free range chickens from a rural area surrounding Funing,China.Fifty-three chickens were antibody-positive and 21 chickens were antigen positive.Hearts,brains,spleens,lungs,livers,and kidneys of 21 antibody or antigen-positive chickens were bioassayed in mice.One strain of T.gondii was isolated from 1 of 21 (4.76%) chickens.The isolated T.gondii killed all of the inoculated mice.Genotyping of this isolate using polymorphisms at the loci 5′-SAG2,3′-SAG2,SAG3,cB21-4,L358,BTUB,and GRA6 revealed that it was Type Ⅰ.These indicated that it was virulent for mice.This is the fnrst report of isolation of T.gondii from chickens in China.
Toxoplasma gondii chickens China isolation
Guangwei Zhao Bo Shen Qing Xie Lixin Xu Ruofeng Yan Xiaokai Song I.A.Hassan Xiangrui Li
College of Veterinary Medicine,Nanjing Agricultural University,210095,China
国内会议
长春
英文
344-344
2012-12-01（万方平台首次上网日期，不代表论文的发表时间）