A DNA vaccine co-expressing Trichinella spiralis MIF and MCD-1 with murine ubiquitin induces partial protective immunity in mice

摘要：

　　Co-expression of Trichinella spiralis macrophage migration inhibitory factor (TsMIF) with T.spiralis cystatin-like domain protein (TsMCD-1) in a DNA vaccine induces a Th1 immune response and partial protection against T.spiralis infection.The present study evaluated whether co-expression of mouse ubiquitin (Ub) with TsMIF and TsMCD-1 might improve the immune response against T.spiralis infection.Groups of BALB/c mice were immunized twice at 2-week intervals with 100 mg of plasmid DNA encoding either a TsMIF-TsMCD-1 fusion protein (pVAX1-Tsmif-Tsmcd-1) or an Ub-co-expressing triple fusion protein Ub-TsMIF-TsMCD-1 (pVAX 1-Ub-Tsmif-Tsmcd-1).

关键词： T.spiralis macrophage migration inhibitory factor cystatin-like domain protein DNA vaccine protective immunity

作者: Fang Tang Lixin Xu Ruofeng Yan Xiaokai Song Xiangrui Li

作者单位: College of Veterinary Medicine,Nanjing Agricultural University,Nanjing,210095,China

会议类型: 国内会议

会议名称: 中国畜牧兽医学会兽医食品卫生学分会第十二届学术研讨会

会议地点: 长春

会议语种:英文

页码: 345-345

在线出版日期: 2012-12-01（万方平台首次上网日期，不代表论文的发表时间）