The Effects of Five Dong Minority Medicinal Plant Extractions on Rat Splenic Lymphocyte Proliferation
Objective:To reveal the rat splenic lymphocyte proliferation induced by the fiveDong minority medicinal plants such as Potentilla discolor Bunge.,Lilhocarpus Polystachys Rehd.,Lonicera macranthoides Hand.-Mazz.,lnula cappa (Buch-Ham.Ex D.Don) DC.and Ampelopsis grossedentata (Hand-Mazz) W.T.Wang.Method:the effects of ultrasonic extractions with 70% ethanol on rat splenic lymphocyte proliferation were investigated by MTT method.The extracts were dissolved by RPMI-1640 medium,and mixed with Lymphocytes,then seeded in 96-well tissue culture plates and incubated at 37℃.Result:the five extracts significantly increased the proliferation of rat splenic lymphocyte.Without ConA synergism,the 800 μg/mL Ampelopsis grossedentata extract exhibited best splenoeytes proliferation effect,the optimal concentrations for Lilhocarpus Polystachys,Potentilla discolor,Lonicera macranthoides and lnula cappa were 800 μg/mL,400 μg/mL,800 μg/mL,400 μg/mL,respectively.With ConA synergism,the optimal concentrations to splenocytes proliferation for Potentilla discolor,Lilhocarpus Polystachys,Lonicera macranthoides,Inula cappa,Ampelopsis grossedentata were 400 μg/mL,100 μg/mL,200 μg/mL,800μg/mL,800 μg/mL,respectively,but the differences between different plant extracts were not obviously.
MTT Assay five medicinal plants ultrasonic extraction with ethanol the splenic lymphocyte proliferation
Xianjin WU Shenghua LI PeiZHANG Hongbo LI
Key Laboratory of Hunan Province for Study and Utilization of Ethnic Medicinal Plant Resources/ Key Laboratory of Hunan Higher Education for western-Hunan Medicinal Plant and Ethnobotany/Department of Life Sciences,Huaihua University,Hunan 418008,China
国内会议
银川
英文
351-352
2012-08-05（万方平台首次上网日期，不代表论文的发表时间）