Over-expression of human TERT C-terminal polypeptide driven by Egr-1 promoter enhances 5-FU induced nasopharyngeal carcinoma growth inhibition and apoptosis

摘要：

　　hTERTC27,a 27 kDa C-terminal polypeptide,can induce telomere dysfunction and inhibit tumor cell proliferation.To study the synergistic anti-tumor effects of hTERTC27 polypeptide driven by Egr-1 promoter and chemotherapeutics 5-flurorouracil (5-FU) on nasopharyngeal carcinoma (NPC),hTERTC27 eDNA was constructed to the downstream of the Egr-1 promoter,and a series of in vitro and in vivo experiments were performed.The results showed that hTERTC27 expression was significantly increased up to 7.21 folds by 5-FU activated Egr-1 promoter in C666-1 cells.Over expressed hTERTC27 made the cells more sensitive to 5-FU,and additionally inhibited cell proliferation about 20.41%.Combinational therapy of over expressed hTERTC27 driven by 5-FU activated Egr-1 promoter and 5-FU synergistically inhibited cell proliferation and promoted apoptosis of C666-1 cells for about 4.75 folds and 1.76 folds in comparison with sole therapy of hTERTC27 or 5-FU in vitro.In vivo experiments showed that over expressed hTERTC27 driven by 5-FU activated Egr-1 promoter and 5-FU synergistically reduced tumor volume,tumor weight and local infiltration,which may be relative to tumor cell apoptosis.These results suggest that combinational therapy of over expressed hTERTC27,which is driven by 5-FU activated Egr-1 promoter,and 5-FU may provide a novel approach to treat nasopharyngeal cancer.

关键词： Egr-1 hTERTC27 5-FU Nasopharyngeal carcinoma gene therapy

作者: Guimiao Lin Wenqi Jiang Marie C.M.Lin Suxia Lin Hong Yao Wanxian Yi Samuel S.M.Ng Xiaomei Wang

作者单位: School of Medical Sciences,Shenzhen University,Shenzhen,518060,China BrainTumor Centre,Division of Neurosurgery,Department of Surgery,The Chinese University of Hongkong,

会议类型: 国内会议

会议名称: 2012医学科学前沿暨第二届个体化治疗与抗肿瘤药物研究新趋向研讨会

会议地点: 深圳

会议语种:英文

页码: 90-107

在线出版日期: 2012-07-13（万方平台首次上网日期，不代表论文的发表时间）