IDH mutation Improves the Prognosis in Chinese Glioma Patients with TP53 Over-expression

摘要：

　　Objective:To investigate the mutational status of isocitrate dehydrogenase 1 (IDH1) and IDH2 of 109 Chinese glioma patients.Methods:Total of 109 Chinese glioma patients was involved in this study.The mutational status of IDH1 and IDH2 of 109 Chinese glioma patients was investigated using immunohistochemical analysis and DNA sequencing.The status of TP53 expression was also investigated using immunohistochemical analysis.Formalin-fixed,paraffin-embedded 5-μm thick tumor tissue sections were used for both DNA isolation and immunohistochemical staining.Anti-human IDH1 R132H and anti-TP53 primary antibodies were used to detect the IDH1 codon 132 mutation (R132H) and TP53 expression,respectively.IDH1 and IDH2 PCR products were sequenced to confirm the presence of IDH1 R132 or IDH2 R172 mutations.

关键词： IDH1 IDH2 TP53 Mutation Glioma Chinese patients

作者: ZENG Jing XIAN Lijian WU Mingwei CHEN Yanfeng ZHANG Jinxin WANG Fang LU Jiabin ZHANG Guanhua LI Zhenye MU Yonggao FU Liwu

作者单位: State Key Laboratory of Oncology in South China,Research Department,Cancer Center,Sun Yat-sen Univer State Key Laboratory of Oncology in South China,Research Department,Cancer Center,Sun Yat-sen Univer State Key Laboratory of Oncology in South China,Research Department,Cancer Center,Sun Yat-sen Univer State Key Laboratory of Oncology in South China,Research Department,Cancer Center,Sun Yat-sen Univer State Key Laboratory of Oncology in South China,Research Department,Cancer Center,Sun Yat-sen Univer

会议类型: 国内会议

会议名称: 2012医学科学前沿暨第二届个体化治疗与抗肿瘤药物研究新趋向研讨会

会议地点: 深圳

会议语种:英文

页码: 435-435

在线出版日期: 2012-07-13（万方平台首次上网日期，不代表论文的发表时间）