HPLC determination of irinotecan and its active metabolite SN-38 in human plasma

摘要：

　　Objective:To develop an HPLC method for the simultaneous determination ofirinotecan(CPT-11) and its active metabolite 7-ethyl-10-hydroxycamptothecin (SN-38) in human plasma.Methods:The internal standard was 10-hydroxycamptothecin.CPT-11 and SN-38 were extracted with ethyl acetate after a complete protein precipitation with 10% perchloric acid,then injected into a Hypersil C18(250 mm×4.6 mm,5um) column; The mobile phase was potassium dihydrogenphosphate (0.1mol·L-1)-acetonitrile (75∶25,adjusted pH3.0 with phosphoric acid);monitored at excitation and emission wavelengths of 355nm and 515nm,respectively.

关键词： HPLC irinotecan SN-38

作者: ZHOU Wang PAN Ying LIAO Hai LIU Tao JIA ShouWei HUANG Hong-bing

作者单位: Department of Pharmacy,Sun Yat-sen University Cancer Center,Guangzhou,510060

会议类型: 国内会议

会议名称: 2012医学科学前沿暨第二届个体化治疗与抗肿瘤药物研究新趋向研讨会

会议地点: 深圳

会议语种:英文

页码: 447-447

在线出版日期: 2012-07-13（万方平台首次上网日期，不代表论文的发表时间）