Long term fertilization affects the ammonia oxidizing community
The abundance and composition of ammonia oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) were investigated from two long term field fertilization experiments (upland red soil and red paddy soils respectively) using quantitative real-time polymerase chain reaction,denaturing gradient gel electrophoresis (DGGE) and constructing clone library based on amoA genes.The soil samples used in this study were collected from the Ecological Experimental Station of Red Soil,Chinese Academy of Sciences,located at Yingtan,Jiangxi Province,China.The long-term fertilizer experiment for upland soil was established with nine treatments including 3 replicates in a randomized plot design:control without fertilizers (CK); chemical fertilizer nitrogen (N); phosphorus (P); potassium (K); combinations of chemical fertilizers NP ; NK ; NPK; 2NPK; and NPK + organic manure (OM).The long-term fertilization experiment for red paddy soil had one more treatment with only organic matter (OM).AOA amoA gene copies outnumbered AOB in all samples.
ammonia oxidation long term field fertilization acidic soils ammonia-oxidizing archaea (AOA) ammonia oxidizing bacteria (AOB) amoAammonia monooxygenase subunit A
M.Saiful Alam Zhongjun Jia
State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science,Chinese Academy o State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science,Chinese Academy o
国内会议
大连
英文
145-147
2012-09-26（万方平台首次上网日期，不代表论文的发表时间）