Difference of Intestine Bacterial Composition of Four Chicken Breeds in Different Feeding Stages
The diversity of bacterial floras in the jejunum,duodenum and cecum of four local chicken breeds from Henan province with a same com diet in two different feeding stages (8 and 48weeks) were examined by analysis of 16S rRNA gene sequences.V3 region of bacterial 16S rRNA gene sequences were detected by denaturing gradient gel electrophoresis (DGGE),and cluster analyses based on the band similarity in migration and intensity between lanes were used to understand the distance of each lanes.The results indicated that only five to eight bands were detected in jejunum and duodenum,and over twenty bands were detected in duodenum.Three intestine position from different breeds were clustered together each,and compared with jejunum and duodenum.Bands appeared in cecum showed much more abundant which indicated more bacterial diversity.
Li Peipei
College of Resources and Environmental sciences,Henan Agricultural University,Zhengzhou 450002,China
国内会议
大连
英文
157-158
2012-09-26（万方平台首次上网日期，不代表论文的发表时间）