Expression of Calretinin in the testes of rats at different development stages
Objectives:Testicular steroidogenesis was mainly regulated by LH secreted by anterior pituitary,while androgen production also subtly regulated by neuroendocrine system and paracrine.Our previous studies showed that the protein profile of human testes changed when endogenous testosterone production was suppressed by exogenous androgen,including many regulators such as calretinin (CALB2).We supposed that CALB2 participated in regulating androgen production in testes.In this study,we observed the different expression of CALB2 in testes of rats at different development stages.Methods:Male SD rats at three development stages (3 to 4 weeks as early maturity group; 16 weeks as middle-aged group; over 12 months as aged group) were used in this study.Sera testosterone concentration was detected by radioimmunoassay (RIA).Cellular location of CALB2 in testes was observed by immunohistochemistry.Expression of CALB2 in mRNA level and protein level was quantificationally analyzed by qPCR and western blot.Results:CALB2 was mainly located in cytoplasm of Leydig cells.Sera testosterone level was low in the rats of early maturity group,and expressions of CALB2 in both mRNA level and protein level were low.Compared to the early maturity group,sera testosterone level in the middle-aged group was significantly higher (P<0.01),and expressions of CALB2 were significantly increased (P<0.05).Interestingly,rats of the aged group had lower sera testosterone level and lower testicular expression levels of CALB2 than the middle-aged group (P<0.05).Conclusion:Calretinin is positively correlated with sera testosterone level,and differently expressed in testes of rats at different development stages,which suggested that calretinin regulated testosterone production as an important paracrine and autocrine factor.
Calretinin testis Leydig cells Testosterone aging
Shan Liu Xiaonan Dai Jing Wang Jian Luo Chao Gao Li Gao Jiayin Liu Yugui Cui
State Key Laboratory of Reproductive Medicine,Clinical Center of Reproductive Medicine,First Affilia State Key Laboratory of Reproductive Medicine,Clinical Center of Reproductive Medicine,First Affilia
国内会议
中华医学会生殖医学分会人类精子库管理学组第三届年会暨全国男性生殖医学和精子库管理新进展第四次研讨会
深圳
英文
173-184
2012-09-06（万方平台首次上网日期，不代表论文的发表时间）