Altered global gene expressions of human embryoid bodies treated with 10-6 mol/L bisphenol A
Objectives:To detect the global gene expressions of human embryoid bodies after disposing by bisphenol A.Methods:Human ES cells were grown in suspension without feeder cells and inhibiting factor bFGF to induce their differentiation into embryoid bodies (EBs).After the EBs were treated with 10-6mol BPA in 7 days,a genechip was utilized to analyze its genes.We used the same way to deal with the EBs in order to get more sample.Using Realtime PCR,to verify Some of the differentially expressed genes.Results:Compare with the control group which dealt with DMSO in 7 days,there were 158 genes upregulated and 458 down-regulated with a threshold fold change over 2.0.Analysis these differentially expressed genes with MAS,a software about Bio-molecular functional annotation system.The results suggested that they were involved in cell differentiation,molecular functions,germinal layer development and signaling pathways.EBs was a powerful tool for leamig human early embryonic development.Treat the EBs with BPA should help us to understand the impact of BPA on early embryonic development and its possible mechanism of action.Conclusion:Differentiation of EBs from human ES cells could be a useful model for dectecing global gene expression changes in response to BPA,which could help us to study the effect or the machanism of BPA on early human embryonic development.
bisphenol A embryoid development differentiation embryoid bodies epigenetics
Luo Jian Liu Jiayin Cui Yugui Dai Xiaonan Jiang Chunyan Huang Boxian Ning Song Chen Aiqin Gao Chao Gao Li
State Key Laboratory of Reproductive Medicine,Clinical Center of Reproductive Medicine,First Affilia State Key Laboratory of Reproductive Medicine,Clinical Center of Reproductive Medicine,First Affilia
国内会议
中华医学会生殖医学分会人类精子库管理学组第三届年会暨全国男性生殖医学和精子库管理新进展第四次研讨会
深圳
英文
193-205
2012-09-06（万方平台首次上网日期，不代表论文的发表时间）