PGD analysis on embryos derived from infertile males with Y chromosome abnormalities
Objective The objectives of this study were to investigate the composition of sex chromosomes in the embryos derived from the infertile males with Y chromosome abnormalities and determine the effects of preimplantation genetic diagnoses (PGD) on the assisted reproductive treatment of these males.Method The couples,of which the infertile male contains Y chromosome abnormalities,were treated with controlled ovarian hyperstimulation and intracytoplasmic sperm injection.In PGD group,single blastomere biopsy was performed and its sex chromosomes were detected by FISH at the third day after fertilization.Female embryo was implanted into the mother”s uterus.In non-PGD group,embryos with normal morphology were implanted.After implantation,the rates of embryo implantation,clinical pregnancy and miscarriage were determined.Results In PGD group,the percentages of female,male and sex chromosome aneuploid embryos were 61.28%,19.92% and 18.80%,respectively.The rates of embryo implantation,clinical pregnancy and miscarriage in PGD group was 33.93%,44.00% and 9.09%,respectively,which was not significantly different with those in the non-PGD group (30.42%,40.95% and 14.29%,respectively).Conclusion Male and aneuploid embryos presents a high percentage in the embryos derived from infertile males with Y chromosomal abnormalities.This indicates that it is necessary to perform PGD for these embryos to avoid the implantation of embryos with genetic defects.Furthermore,PGD does not have significant effects on the implantation,embryo development and the assisted reproductive treatment.Therefore,PGD is necessary and feasible for the assisted reproductive treatment of infertile males with Y chromosome abnormalities.
Preimplantation genetic diagnoses (PGD) Fluorescence in situ hybridization (FISH) Male infertility
Jun Wang Yaning Luo Yefei Ma Li Li Jianlei Huang Xiaohong Wang
Center for Reproductive Medicine,Tang Du Hospital,Fourth Military Medical University,Xi”an,710038,China
国内会议
中华医学会生殖医学分会人类精子库管理学组第三届年会暨全国男性生殖医学和精子库管理新进展第四次研讨会
深圳
英文
265-266
2012-09-06（万方平台首次上网日期，不代表论文的发表时间）