A protocol for extraction of high-quality RNA and DNA from peanut plant tissues

摘要：

　　Peanuts are an increasingly important global food source.However,until recently the lack of effective protocols for the extraction of nucleic acids has made molecular studies of peanut development and maturation difficult.Here we describe a method to isolate high-quality RNA and DNA from peanut tissue and have successfully applied this method to peanut plant roots,stems,leaves,flowers,and seeds.Spectrophotometric analysis showed that the average yields of total RNA from 100 mg of peanut materials ranged from 24.52 μg to 74.6 μg,and those ofgenomic DNA from the same tissues ranged from 23.47 μg to 57.68 μg.

作者: Dongmei Yin Xingguo Zhang Yun Wang Dangqun Cui

作者单位: Henan Agricultural University, 450002, Zhengzhou, China

会议类型: 国内会议

会议名称: 2012遗传学进步促进粮食安全与人口健康高峰论坛

会议地点: 郑州

会议语种:英文

页码: 2-2

在线出版日期: 2012-08-13（万方平台首次上网日期，不代表论文的发表时间）