Haematococcus pluvialis-Based High-throughput Screening for Enhanced Activity of Atrazine Chlorohydrolase by Directed Evolution

摘要：

　　The atrazine chlorohydrolase (AtzA) attracts widespread interests as it catalyzes the toxic atrazine to non-toxic hydroxyatrazine and can be used in the biodegradation of atrazine.To facilitate this application,we developed a Haematococcus pluvialis-based high-throughput method to screen AtzA mutants from random mutagenesis libraries.Eight variants with enhanced enzyme activity were successfully obtained.All variants showed significant increase in their specific activity,ranging from 2.2-to 5.1-fold compared to the wild-type.

作者: Yi-Cun Wang Xiao-Long Li Hai-Wei Chen Xi-Wen Chen De-Fu Chen

作者单位: Laboratory of Molecular Genetics, College of Life Sciences, Nankai University, Tianjin, China Laboratory of Molecular Genetics, College of Life Sciences, Nankai University, Tianjin, China;Colleg

会议类型: 国内会议

会议名称: 2012遗传学进步促进粮食安全与人口健康高峰论坛

会议地点: 郑州

会议语种:英文

页码: 133-133

在线出版日期: 2012-08-13（万方平台首次上网日期，不代表论文的发表时间）