Analysis of cellular proteome alterations in porcine alveolar macrophage cells infected with 2009 (H1N1) and classical swine H1N1 influenza viruses
The H1N1/2009 influenza virus has the potential to cause a human pandemic,and sporadic cases of human-to-pig transmission have been reported.In this study,two influenza viruses were isolated from pigs.A phylogenetic analysis showed that the A/swine/NanChang/F9/2010(H1N1) (F9/10) strain shared a high degree of homology with the pandemic H1N1/2009 virus,and A/swine/GuangDong/34/2006 (H1N1) (34/06) strains was a classical swine influenza virus.A proteomic analysis was performed to investigate possible alterations of protein expression in porcine alveolar macrophage (PAM) cells infected by the F9/10 and 34/06 viruses over different time courses.Using 2-DE in association with MALDI-TOF MS/MS,we identified 13 up-regulated and 21 down-regulated protein spots,including cytoskeleton proteins,cellular signal transduction proteins,molecular biosynthesis proteins and heat shock proteins.
JiPing Zhu Wei Zou GuangMin Jia HongBo Zhou Yong Hua MengYun Peng HuanChun Chen MeiLin Jin
State Key Laboratory of Agriculture Microbiology,Huazhong Agriculture University,Wuhan 430070,PR China
国内会议
武汉
英文
8-8
2012-07-13（万方平台首次上网日期，不代表论文的发表时间）