Multiple genes encoding fluoroquinolone resistance co-located with ESBL genes in non-typhoidal salmonella isolated from food producing animals
OBJECTIVES To investigate the molecular epidemiology of genes that confer resistance to fluoroquinolones and extended spectrum β-lactams in non-typhoidal Salmonella(NTS)from food producing animals in China.METHODS Thirty-one non-duplicate NTS were examined by PCR for plasmid mediated ESBL(TEM,SHV,OXA,CMY,and CTX-M-9),fluoroquinolone-resistance(qnr,aac(6”)-Ib-cr,qepA and oqx)genes.The QRDR of topoisomerase genes (gyrA,gyrB,parC and parE)was also sequenced.Genetic relatedness of isolates was determined by PFGE;susceptibility to antibiotics was measured with CLSI methods.RESULTS Numerous combinations of different antibiotic resistance genes and mutations in topoisomerase genes were detected.Of most concern was that different isolates of Salmonella each contained several antibiotic resistance genes which together conferred high level resistance to extended spectrum β3-lactams and fluoroquinolones.CONCLUSIONS These data suggest that contamination of food by multi-drug resistant bacteria in exporting countries with highly mobile populations will lead to global transfer of new antibiotic resistances from country to country by humans or on food.Human infections by such strains would be hard to treat.
plasmid Salmonella fluoroquinolone ESBL
Xiao-Ping Fu Yu-Fang Guo Li-Hua Wang Jun-Yu Ren Hong-Xia Jiang
College of Veterinary Medicine,National Reference Laboratory of Veterinary Drug Residues,South China Agricultural University(SCAU),Guangzhou 510642,China
国内会议
贵阳
英文
288-288
2012-07-01（万方平台首次上网日期，不代表论文的发表时间）