Silencing of molt-regulating transcription factor gene, CiHR3 affects growth and development of sugarcane stem borer, Chilo infuscatellus Snellen (Crambidae:Lepidoptera)
RNA interference (RNAi) is a breakthrough technology for conducting functional genomic studies and a potential tool for crop protection against insect pests.Development of reliable methods for production and delivery of double-stranded RNA(dsRNA) is the major challenge for efficient pest control.In this research,Chilo infuscatellus Snellen was fed CiHR3 dsRNA expressed in bacteria or synthesized in vitro.The dsRNA absorbed by Chilo infuscatellus Snellen successfully triggered silencing of the molt-regulating transcription factor CiHR3,an important gene for insect growth and development,caused significant abnormal and weight loss in one week.This study is a good example of feeding-based RNAi mediated by dsRNA expressed in bacteria or synthesized in vitro.The results also suggested that feeding-based RNA interference is a potential method for the management of Chilo infuscatellus Snellen.Function study of genes related to insect molting is not only helpful in understanding the insect molting mechanism,but also helpful in screening out new biological control targets.
Chilo infuscatellus Snellen RNA interference bacteria-expressed dsRNA CiHR3 bioassay
Yu-liang Zhang Shu-zhen Zhang Mahesh Kulye Su-ran Wu Nai-tong Yu Jian-hua Wang Hong-mei Zeng Zhi-xin Liu
Key Laboratory for Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Biosc Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant P
国内会议
厦门
英文
61-72
2012-07-01（万方平台首次上网日期，不代表论文的发表时间）