Development of high-resolution melting analysis for the detection of the MYD88 L265P mutation
Objective Recurrent L265P mutation of myeloid differentiation primary response gene 88 (MYD88) has been identified in a proportion of diffuse large B-cell lymphoma (DLBCL) and chronic lymphocytic leukemia.The present study was aimed to establish a rapid,sensitive,and reliable method using high-resolution melting analysis (HRMA) to detect MYD88 L265P mutation in DLBCL.Methods The sensitivity of HRMA in the detection of MYD88 L265P mutation was evaluated.MYD88 L265P mutation was further screened in 120 patients with DLBCL.The results of HRMA were validated by direct DNA sequencing.Results For the detection of MYD88 L265P mutation,the reproducible maximal sensitivity of HRMA was 5%,higher than that obtained by direct DNA sequencing (25%).Heterozygous MYD88 L265P mutations were identified in 11 (9.2%) DLBCL cases,all of which were diagnosed as non-germinal-center B cell (non-GCB) DLBCL.Conclusions The HRMA assay is a rapid,sensitive,reliable,and high-throughput method to screen MYD88 L265P mutation and could be used in clinical diagnostic laboratories.
MYD88 Mutation High-resolution melting analysis Diffuse large B-cell lymphoma
Wang Cui-zhu Li Yun Chen Xing-xing Qian Jun Lin Jiang Shao Rui Xue Di Qian Wei Xiao Gao-fei Deng Zhao-qun Yang Jing
Affiliated People”s Hospital of Jiangsu University
国内会议
三亚
英文
152-152
2012-11-16(万方平台首次上网日期,不代表论文的发表时间)