A Combination of Ultrasound-Assisted Extraction with High-Speed Counter-Current Chromatography for the Preparation of 4-trans-l0-Hydroxy-2-Decenoic Acid (10-HDA) from Royal Jelly

摘要：

　　An efficient method was built successfully for the rapid separation and purification of 4-trans-l0-hydroxy-2-decenoic acid (10-HDA) from royal jelly by ultrasound-assisted extraction (UAE) in combination with high-speed counter-current chromatography (HSCCC).The UAE conditions including percent ethanol,extraction time and solvent ratio were optimized with an orthogonal test.The 10-HDA in the crude extract was separated and purified by HSCCC with a two-phase solvent system composed of petroleum ether-aether-methanol-water (1∶1∶0.5∶1.5,v/v).98.2 mg of 10-HDA was obtained from 1.0 g of crude extract in one-step separation with the purity of 99.1% as determined by HPLC.The chemical structure of 10-HDA was identified by ESI-MS,1H-NMR and 13C-NMR.

关键词： Royal jelly 10-HDA Ultrasound-assisted extraction High-speed counter-current chromatography

作者: Xiaojing Lin Xikai Shu Yan-ling Geng Feng Liu Jian-hua Liu Xiao Wang

作者单位: Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street,Jinan, Shandong 25 Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street,Jinan, Shandong 25

会议类型: 国内会议

会议名称: 第二届环渤海色谱学术报告会

会议地点: 青岛

会议语种:英文

页码: 973-984

在线出版日期: 2012-09-19（万方平台首次上网日期，不代表论文的发表时间）