UHPLC-MS/MS法测定猴血浆中艾塞那肽浓度
A highly sensitive ultra-high-performance liquid chromatographic-tandem mass spectrometry method (UHPLC/MS/MS) was developed for the quantification of the synthetic peptide drug of exenatide in monkey plasma. Sample preparation was carried out by solid-phase extraction (SPE)，and bivalirudin was used as the internal standard (I. S.).An excellent chromatographic separation was obtained on a reversed-phase C18 column with a gradient elution. Detection utilized a Qtrap 5500 μL system operated in the positive ion mode with multiple reaction monitoring (MRM).The proposed method was validated by assessing the specificity, linearity, intra-and inter-day precision and accuracy, recovery, and stability. The method resulted in a linear calibration range of 0. 10-30 ng/mL, extracting with only 50 monkey plasma aliquots. The intra- and inter-day precision (as relative standard deviation)were less than 7.5% and 9.6%, respectively. The method was validated and could be successfully utilized for the pharmacokinetic study of exenatide in monkeys following a single subcutaneous injection of Byetta.
艾塞那肽 药物浓度 血浆分析 动物实验
张金凤 Jinfeng Zhang Chunjie Sha 沙春洁 Yu Sun 孙瑜 Jiye Sun 孙吉叶 Yunyun Gai 盖芸芸 Jiangbin Han 韩江斌 Xin Shao 邵馨 Chunna Sha 沙春娜 李又欣 Youxin Li Wanhui Liu 刘万卉
烟台大学药学院,山东 烟台 264003 School of Pharmacy,Yantai University,Yantai 264005,P.R.China State Key Laboratory of Long-acting and Targeting Drug Delivery System,Shandong Luye Pharmaceutical 山东绿叶制药有限公司,山东 烟台 264003 Rushan People”s Hospital,Weihai 264200,P.R.China 乳山人民医院,山东 威海 264200 School of Pharmacy,Yantai University,Yantai 264005,P.R.China;State Key Laboratory of Long-acting and 烟台大学药学院,山东 烟台 264003;山东绿叶制药有限公司,山东 烟台 264003
国内会议
青岛
中文
480-485
2012-09-19（万方平台首次上网日期，不代表论文的发表时间）