Partial Tolerance Induced by Transplantation of Spatially Separated Thymuses:A Cue for T Cell Retolerization in Thymus Grafts
Thymus grafts made up of mixed syngeneic and xenogeneic thymus tissues could induce donor-specific tolerance to xenografis with no development of autoimmune syndrome (AIS).But the requirements for the simultaneous presentation of tissue antigens from both species in the process of T cell development in thymus grafts have not hitherto been defmed.To do this,we setup a model in which xenothymus grafts from F344 rats were heterotopically implanted into BALB/c nude mice carrying syngeneic thymus grafts,and the grafts were either mixed together or spatially separately; next,we examined the induction of donor-specific tolerance,any pathological changes and the distribution of T lymphocytes.In contrast to the mixed thymus grafts,spatially separated thymus transplants could neither induce a long-term tolerance to skin grafts nor prevent AIS completely.51Cr-labelled cell-tracing experiments showed that mature peripheral T cells could re-enter into both kinds of thymus grafts,while the T cells isolated from the syngeneic thymus tended to concentrate in the xenothymus grafts.Hence,our data suggest that the immune tolerance induced by mixed thymus transplants could be partially reversed if the thymus tissues from donors and recipients were segregated by spatial telorism.The uneven recirculation of mature T cells might imply that T cells get retolerized within the thymus grafts.
吴军 杨思思 杨俊杰 姚志慧 宋华培 刘岱松 武艳军 张小容 胡晓红 周峻峄 王锡华 罗高兴 解志杰 贺伟峰 崔艳艳 谭江玲 王晓娟
第三军医大学西南医院烧伤研究所;创伤、烧伤与复合伤国家重点实验室;重庆市疾病蛋白质组学重点实验定 重庆400038
国内会议
广州
英文
372-373
2012-08-30（万方平台首次上网日期，不代表论文的发表时间）