Expression imbalance of transcription factors GATA-3 and T-bet in post-resuscitation myocardial dysfunction in a porcine model of cardiac arrest

摘要：

　　Background:This study investigated whether an imbalance in Th1/Th2 ceils is involved in the post-resuscitation myocardial dysfunction.Methods:After pigs were subjected to 8 min of electrically-induced cardiac arrest,animals in the post-return of spontaneous circulation (ROSC) group were randomly assigned to be sacrificed (n =8 per group) at 12 and 24 h after ROSC.CD4+ and CD8+ lymphocyte subsets were determined by flow cytometry,IL-4 and IFN-γ in the myocardium were measured by ELISA,.and protein and mRNA levels of GATA-3 and T-bet were detected in the myocardium by western blotting and quantitative real-time PCR,respectively.

关键词： Th1/Th2 imbalance cardiopulmonary resuscitation postresuscitation myocardial dysfunction GATA-3 T-bet

作者: Wei Gu ChunSheng Li WenPeng Yin XiaoMin Hou Jian Zhang Da Zhang ZhiJun Guo

作者单位: Department of Emergency Medicine,Beijing Chaoyang Hospital, Capital Medical University,Beijing, China

会议类型: 国内会议

会议名称: 2012北京医学会急诊医学分会学术年会

会议地点: 北京

会议语种:英文

页码: 122-122

在线出版日期: 2012-12-01（万方平台首次上网日期，不代表论文的发表时间）