Application of microdialysis for elucidating the existing form of hyperoside in rat brain:comparison between intragastric and intraperitoneal administration
Ethnopharmacological relevance:Hypericum perforatum (St.John”s wort) is an important antidepressant herb used in clinic and commonly prescribed for mild depression.Hyperoside is one of the major components of Hypericum perforatum and is also detected in many plant species such as Abelmoschus manihot,Black Currant,Rosa agrestis,Apocynum venetum and Nelumbo nucifera.Aim of the study:As hyperoside showed CNS (central nervous system) protective activity (e.g.antidepressant-like effect),the possibility of hyperoside or its metabolites to reach CNS should be investigated.Moreover,the pharmacokinetics profile of hyperoside or its metabolites in rat brain should be studied for further elucidating the mechanism of hyperoside action on CNS.Material and Methods:A simple method for simultaneous determination of unbound hyperoside and its metabolite 3′-O-methyl-hyperoside in rat brain was developed by using ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) and microdialysis technique.
hyperoside 3′-O-methyl-hyperoside UPLC-MS/MS microdialysate
Jian-ming Guo Ping Lin Jin-ao Duan Er-xin Shang Da-wei Qian Yu-ping Tang
Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese M Jiangsu SZYY Group Pharmaceutical Limited, Nanjing 210016, PR China
国内会议
海口
英文
267-268
2012-11-01（万方平台首次上网日期，不代表论文的发表时间）