Short sequence and secondary structure of ITS2, compared to the full length ITS, provide efficient taxonomic sequence tags
An ideal DNA barcoding region should be short enough to amplify from degraded DNA.In this article,we discuss the possibility of mini nuclear DNA sequence in identifying plant species.Firstly,the PCR and sequencing recovery of ITS and ITS2 were evaluated based entirely on materials from dry medicinal product and herbarium voucher specimens with some samples collected 90 years ago.The results showed that ITS2 could recovered 91% while ITS only 23% efficiency of PCR and sequencing by using one pair of primer.Secondly,the ITS and ITS2 sequences of 12862 plant samples including 8303 species,were downloaded from GenBank used for comparing the identification efficiency.
ITS region ITS2 Sequence-structure monophyletic
Jianping Han Yingjie Zhu Linchun Shi Chang Liu Hui Yao Jingyuan Song Shilin Chen
Institute of Medicinal Plant Development, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, P.R.China
国内会议
海口
英文
397-397
2012-11-01（万方平台首次上网日期，不代表论文的发表时间）