Simultaneous determination of triterpenoid saponins in dog plasma by a validated UPLC-MS/MS and its application to a pharmacokinetic study after administration of total saponin of licorice
A rapid and sensitive ultra performance liquid chromatography-tandem mass spectrometry method was developed for simultaneous determination of eight constituents,uralsaponin C,uralsaponin F,22β-acetoxyl-glycyrrhizin,24-hydroxy-licorice-saponin E2,lieorice-saponin G2,licorice-saponin E2,glycyrrhizin,and licorice-saponin J2,from total saponin of licorice (TSL) in dog plasma.Ardisiacrispin A was used as the internal standard (IS).The separation was performed on Thermo Syncronic C18 column (100 mm×2.1 mm,1.7 μm) at a flow rate of 0.4 mL min-1,and acetonitrile/methanol (3∶1,v/v)-0.1% formic acid was used as mobile phase.The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM)via electrospray ionization (ESI) source with negative ionization mode.All calibration curves had good linearity (r > 0.991) over the concentration range from 2.03-405 ng mL-1 to 2.63-2625 ng mL-1 for all components.The intra-and inter-day precision was within 15% and the accuracy ranged from-14.08 % to 13.8%.The method was successfully applied to pharmacokiuetic study of eight triterpenoid saponins in dog plasma after oral administration of TSL.
Licorice Triterpenoid saponins UPLC-MS/MS Dog plasma Pharmacokinetic
Weiwei Taoa Jinao Duan Jianming Guo Jianping Lia Yuping Tanga Pei Liu Nianyun Yang
Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China
国内会议
海口
英文
408-409
2012-11-01(万方平台首次上网日期,不代表论文的发表时间)