Protein Microarray Analysis in a Mouse Cecal Ligation and Puncture Model for Early Prediction of Sepsis-Induced Disseminated Intravascular Coagulation
Objective To identify valuable biomarkers of sepsis-induced disseminated intravascular coagulation (DIC) from platelets-derived factors by using a biotin label-based custom protein microarray technology in a mouse cecal ligation and puncture (CLP) model.Methods KMmice were randomized into sham-operated group and CLP group.Blood were detected immediately,at 1hr,2 hrs,6 hrs,12 hrs,24 hrs,48 hrs and 72 hrs after CLP for platelet count,coagulation assay and blood chemistries.Histological assessment of the lung and mesenterium tissue was executed at all corresponding time points for microthrombus formation.Serial detection by protein microarray analysis was performed for platelets-derived factors.Results The survivor probability at the time point of 72hrs post-CLP was 15% and there was no mortality among the sham mice.
Jingchun Song Zhaofen Lin Zili Chen Dunzhong Hu Chao He Tao Wang Xuefeng Liu Xi Wang Lv Wang Linhao Ma Jinlong Qu
Department of emergency and critical care medicine,the 94th Hospital of PLA,Nanchang,Jiangxi Provinc Department of emergency and critical care medicine,Shanghai Changzheng Hospital,The Second Military
国内会议
天津
英文
60-61
2012-10-20（万方平台首次上网日期，不代表论文的发表时间）