Cross talk of NF-kappa B canonical and non-canonical pathway in human and mouse tissues
Background:Nuclear factor-kappa B (NF-κB) family are critical regulators in immunity,stress responses,apoptosis and differentiation,however,tissue NF-κB metabolism and their relevance to methylation status remain unknown.Method:We examined gene expression profiles of 5 NF-κB transcription factors,6 inhibitory IκB proteins (I-κB),3 IκB kinase (IKK) complex and 24 pathway components in NF-κB signaling pathway.Data-base mining of NF-κB family members and conponnets mRNA expressions are accomplished by querying GEO DataSets profiles under inflammation cytokine stimuli,hypomethylation status,and homocysteine treat.We analyzed correlations between gene expression,S-adenosylhomocysteine (SAH),and S-adenosylmethion ine(SAM) levels,and SAM/SAH ratios in 7 mouse tissues.Result:Our results showed that 1) NF-κB family members and pathway components have different expression levels in different tissues,and high expression in mouse,but inactive in human in normal condition;2) Vascular system has lower response to NF-κB activity;3) Tissues can be divided into 3 tiers according to essential molecular of NF-κB canonical and non-canonical pathway expression level in normal condition;4) Canonical pathway is activated immediately after inflammatory cytokine TNF-α,IL-1 stimuli,but non-canonical pathway is up-regulated until 4 hours treatment;5) Lymphotoxin beta/LIGHT has no effect on NF-κB family members and components expressions at 4 hours;6) Scal+ subset endothelial progenitor cells has lower NF-κB family members and components expressions level than Scalsubset endothelial progenitor cells;7) Most of NF-κB family members and components expressions are down-regulated by hypomethylation status;8) Most of NF-κB family members and components expressions are down-regulated by acute homocystein treat in different cell type.
Xiao Huang Ren Gong Xiao-feng Yang Hong Wang Xiao-shu Cheng
Department of Cardiology,Second hospital of Nanchang University,Nan Chang,Jiang Xi,China 330006;Depa Department of Pharmacology and Cardiovascular Research Center,Temple University School of Medicine,P Department of Cardiology,Second hospital of Nanchang University,Nan Chang,Jiang Xi,China 330006
国内会议
天津
英文
521-522
2012-10-20（万方平台首次上网日期，不代表论文的发表时间）