利用基于SNP分析的单管PCR法检测肺炎支原体耐药基因突变
Mycoplasma pneumoniae is one of the common pathogens of atypical pneumoniae,it is usually sensitive to macrolides.However,in recent years,resistance to the macrolides has occurred in M.pneumoniae,which appears to be increasing year by year.The rise in macrolides resistance in M.pneumoniae poses a major challenge to M.pneumoniae pandemic preparedness. It is confirmed that the macrolides resistance is caused by the mutations on domain V of 23S rRNA gene of M.pneumoniae in previous studies.Sodevelopment of rapid sensitive resistance monitoring methods becomes time-critical and relevant globally.In this study,we developed a faster and least expensive method using PCR-based single nucleotide polymorphism (SNP) analysis to detect macrolides resistantmutations from throat swabs.
肺炎支原体 耐药基因突变 单管PCR法 SNP分析
李少丽 孙红妹 曹玲 袁艺 赵汉青 冯燕玲 薛冠华
首都儿科研究所细菌研究室 100020
国内会议
北京
中文
117-118
2012-06-09（万方平台首次上网日期，不代表论文的发表时间）