Simultaneous determination of 3,3”,4”,5,7-pentamethylquercetin and its possible metabolite 3,3”,4”,7-tetramethylquercetin in dog plasma by liquid chromatography-tandem mass spectrometry and its application to preclinical pharmacokinetic study
A sensitive,simple and rapid ultra fast liquid chromatography (UFLC)-ESI-MS/MS method was established for the simultaneous determination of 3,3”,4”,5,7-pentamethylquercetin (PMQ) and its possible metabolite 3,3”,4”,7-tetramethylquercetin (TMQ) in dog plasma using 4”,5,7-trimethylapigenin (TMA) as the internal standard.The plasma sample was pretreated with acetonitrile for protein precipitation and the analytes were separated on an Ultimate XB-CN column (5μm,2.1mm× 150mm) with the mobile phase consisting of acetonitrile and water (2∶1,v/v).Detection was performed on a triple-quadrupole tandem mass spectrometer under a positive multiple reaction-monitoring mode (MRM).The mass transition ion-pair was followed as m/z 373.1-312.1 for PMQ,359.1-344.0 for TMQ and 313.1-298.1 for TMA.The validated concentration ranged from 1.272 to 3060ng/mL for PMQ and from 10.35 to 1725 ng/mL for TMQ.The lower limit of quantifications for PMQ and TMQ were 1.272 ng/mL and 10.35ng/mL respectively.The developed-method was successfully applied for the pharmacokinetic study of PMQ and its metabolite TMQ in dogs following a single oral dose.
3,3”,4”,5,7-pentamethylquercetin 3,3”,4”,7-tetramethylquercetin LC-MS/MS Pharmacokinetic
Xiaomei Chen Dongyan Li Yan Hu Manwen Jin Liping Zhou Kelong Peng Heng Zheng
Department of Pharmacy, Tongji Hospital of Tongji Medical College, Huazhong University of Science an Department of Pharmacology, School of Basic Medical Sciences, Tongji Medical College, Huazhong Unive Department of Pharmacy, Tongji Hospital of Tongji Medical College, Huazhong University of Science an
国内会议
成都
英文
81-87
2012-10-26（万方平台首次上网日期，不代表论文的发表时间）