Simultaneous Determination of Midazolam and its Metabolite 1”-Hydroxymidazolam in Rat Plasma by LC-MS
A sensitive and selective liquid chromatography-mass spectrometry (LC-MS) method for determination of midazolam and its metabolite l”-hydroxymidazolam in rat plasma was developed and validated.We take the method of acetonitrile precipitation to extracted the analytes and internal standard carbamazepine from plasma.The chromatographic separation was performed on a Zorbax SB-C18 column (150 × 2.1□mm,5 □ μm),using acetonitrile-0.1% formic acid as the mobile phase with gradient elution,delivered at a flow-rate of 0.4 ml/min.Electrospray ionization (ESI) source was applied and operated in positive ion mode,and selected ion monitoring (SIM) mode used to quantify midazolam and its metabolite l”-hydroxymidazolam.Calibration curves were linear in the concentration ranges of 5-2000 ng/mL for midazolam and 10-2000 ng/mL for 1 ”-hydroxymidazolam,with a lower limit of quantification (LLOQ) of 5 ng/mL for midazolam and 10 ng/mL for 1 ”-hydroxymidazolam.Intra-and inter-day precision were less than 13% and the accuracy ranged from-10.7% to 9.5%.This developed method was successfully used for determination of midazolam and metabolite l”-hydroxymidazolam in rat plasma for pharmacokinetic study.
LC-MS midazolam 1”-hydroxymidazolam plasma
Xiaole Chen Jianchun Pan Tao Xu Zhe Wang Peipei Pan Shunbing Luo Dan Lin Guoxin Hu Jianshe Ma Xianqin Wang
Shanghai Key Laboratory of Forensic Medicine(Institute of Forensic Science, Ministry of Justice), Sh School of Pharmacy of Wenzhou Medical College, Wenzhou 325035, China Function Experiment Teaching Center of Wenzhou Medical College, Wenzhou 325035, China.
国内会议
成都
英文
106-107
2012-10-26（万方平台首次上网日期，不代表论文的发表时间）