Simultaneous Determination of Naphthoquine phosphate, and Azithromycin in Human Plasma by HPLC-MS-MS
A rapid,sensitive and specific assay method has been developed to simultaneously determine human plasma concentrations of naphthoquine and azithromycin,using high-performance liquid chromatography with an electrospray ionization(ESI) tandem mass spectrometry (HPLC-MS-MS).Chromatographic separation was performed on an Agilent-Zorbax-Eclipse-XDB-C18 column(75mm×2.lmm,3.51μm,Agilent,USA) using a gradient mobile phase with ultrapure water containing 0.1% formic acid and methanol by gradient elution.Detection and quantitation were performed by MS/MS using electrospray ionization and multiple reactions monitoring in the positive ion mode.The calibration curves were linear over the concentration ranges 0.25-100 ng/mL for naphthoquine (r=0.9990) and 1-1000 ng/mL for azithromycin (r =0.9974).The limit of quantification was 0.25ng/mL for naphthoquine and 1 ng/mL for azithromycin.The relative recovery was 99.97±4.89,96.97±3.52 and 100.02±2.27% for naphthoquine,110.13±7.83,88.42±1.67 and 102.13±2.22% for azithromycin.The method was accurate,sensitive and simple and was successfully applied to a pharmacokinetic study after a single oral administration of the combination at a dose of 400 mg in 12 healthy Chinese volunteers.
HPLC-MS-MS Naphthoquine Azithromycin
Yuanyuan Li Guangtao Hao Hongzhi Gao Hengyan Qu Zeyuan Liu
Department of Pharmacology, Affiliated Hospital of the Academy of Military Medical Sciences, No.8, East Street, Fengtai District, Beijing 100071, PR China
国内会议
成都
英文
267-272
2012-10-26（万方平台首次上网日期，不代表论文的发表时间）