Quantification of exenatide in human plasma using high-resolution/accurate mass LC-MS/MS
Objective:Exenatide is a polypeptide containing 39 amino acids.It”s a synthetic glucagon-like peptide (GLP-1)analog,and has a high affinity to GLP-1 receptor,clinically used for the treatment of type 2 diabetes.Conventional analysis method for quantification of exenatide is immunoassay technique,but their specificity,stability and reproducibility is poor,and the method is expensive.LC-MS is a rapidly development method for drug analysis in biological matrix in recent years.However,the low plasma concentration of exenatide and interference from endogenous substances bring a huge challenge for the evaluation of its pharmacokinetic characteristics.Triple TOF is a new mass spectrometer with high-sensitivity,high-resolution and high-quality accuracy under maximum scanning speed.It”s able to provide similar MRM quantitative ability of high-resolution mass spectrometry,the lowest limit of detection and dynamic range can be quite high-end triple-quadrupole mass spectrometry system.
Wang Jing Yang Yan Cheng Longmei Gu Jingkai
Research Center for Drug Metabolism, College of Life Science, Jilin University, Changchun 130012, Ch Clinical Pharmacology Center, Transformation medical institute, the First Bethune Hospital of Jilin
国内会议
成都
英文
355-355
2012-10-26（万方平台首次上网日期，不代表论文的发表时间）