Identification of a novel species ”Mycobacterium fukienense” sp.nov of Mycobacterium chelonae/abscessus complex from tuberculosis patients in Fujian Province,China
Clinical M.chelonae/abscessus complex strains collected from provinces in China were identified by phenotypic tests.Members were verified by Conventional biochemical tests and sequencing of 16s rRNA gene.Species and subspecies were identified by hsp65 and rpoB PCR-RFLP methods.In total,27 clinical specimens were identified as Mycobacterium chelonae/abscessus complex by phenotypic tests.16s rRNA gene sequence analysis of all 27 clinical samples shared over 99.7％ similarity with M.Chelonea and M.abscessus.Species identification with hsp65 PCR-RFLP and rpoB PCR-RFLP revealed that 18 specimens were M.abscessus and 4 were M.absecces,but the remaining 5 isolates displayed a pattern of rpoB PCR-RFLP that failed to match any previously reported pattern.
Species identification Mycobacterium chelonae/abscessus complex Mycobacterium fukienense
Yuan-yuan Zhang Yan-bing Li Ming-xiang Huang
State Key Laboratory for Infections Disease Prevention and Control / National Institute for Communic State Key Laboratory for Infections Disease Prevention and Control / National Institute for Communic Fuzhou pulmonary Hospital/Clinical Teac
国内会议
长沙
英文
64-79
2012-09-17（万方平台首次上网日期，不代表论文的发表时间）