会议专题

Preparative isolation of imperatorin,oxypeucedanin and isoimperatorin from a traditional Chinese herb using a high-speed countercurrent chromatography strategy based on optimization of rapid flow rate

Preparative high-speed countercurrent chromatography has been used successfully for the isolation and purification of imperatorin,oxypeucedanin and isoimperatorin from traditional Chinese herb ”bai zhi”-Angelica dahurica (Fisch.ex Hoffm) Benth.et Hook by using high-speed countercurrent chromatography (HSCCC).This was achieved in two stages.The first stage used a high flow HSCCC protocol with a two-phase solvent systems composed of n-hexane-ethyl acetate-methanol-water (HEMW)with volume ratios of 5∶5∶5∶5,v/v which isolated isoimperatorin but co-eluted imperatorin & oxypeucedanin.The second stage used HEMW 5∶5∶4∶6,v/v at low flow rate to resolve the co-eluted components from the first stage.The flow rate was optimized using preparative HSCCC.300 mg amount of the crude extract was separated,yielding 18.5 mg of imperatorin,8.3 mg of oxypeucedanin and 9.8 mg of isoimperatorn all at a high purity of over 98%.

high-speed countercurrent chromatography imperatorin oxypeucedanin isoimperatorin Angelica dahurica (Fisch.ex Hoffm) Benth.et Hook

Yun Wei Qianqian Xie Derek Fisher Ian A.Sutherland

Applied Chemistry Department,Faculty of Sciences,Beijing University of Chemical Technology,15 Beisan Advanced Bioprocessing Centre,Brunel Institute for Bioengineering,Brunel University,Uxbridge,Middles Advanced Bioproc

国内会议

第五届全国化学工程与生物化工年会

西安

英文

23-32

2008-10-01(万方平台首次上网日期,不代表论文的发表时间)