Preparative isolation of imperatorin,oxypeucedanin and isoimperatorin from a traditional Chinese herb using a high-speed countercurrent chromatography strategy based on optimization of rapid flow rate
Preparative high-speed countercurrent chromatography has been used successfully for the isolation and purification of imperatorin,oxypeucedanin and isoimperatorin from traditional Chinese herb ”bai zhi”-Angelica dahurica (Fisch.ex Hoffm) Benth.et Hook by using high-speed countercurrent chromatography (HSCCC).This was achieved in two stages.The first stage used a high flow HSCCC protocol with a two-phase solvent systems composed of n-hexane-ethyl acetate-methanol-water (HEMW)with volume ratios of 5∶5∶5∶5,v/v which isolated isoimperatorin but co-eluted imperatorin & oxypeucedanin.The second stage used HEMW 5∶5∶4∶6,v/v at low flow rate to resolve the co-eluted components from the first stage.The flow rate was optimized using preparative HSCCC.300 mg amount of the crude extract was separated,yielding 18.5 mg of imperatorin,8.3 mg of oxypeucedanin and 9.8 mg of isoimperatorn all at a high purity of over 98％.
high-speed countercurrent chromatography imperatorin oxypeucedanin isoimperatorin Angelica dahurica (Fisch.ex Hoffm) Benth.et Hook
Yun Wei Qianqian Xie Derek Fisher Ian A.Sutherland
Applied Chemistry Department,Faculty of Sciences,Beijing University of Chemical Technology,15 Beisan Advanced Bioprocessing Centre,Brunel Institute for Bioengineering,Brunel University,Uxbridge,Middles Advanced Bioproc
国内会议
西安
英文
23-32
2008-10-01（万方平台首次上网日期，不代表论文的发表时间）