The Melanocortin 1 Receptor (MC1R) Inhibits the Inflammatory Response in Raw 264.7 Cells
(0) The alpha melanocyte stimulating hormone receptor (MC1R) is one of five G-protein coupled receptors belonging to the melanocortin subfamily,mclr gene has been known to play a major role in regulating of fur color in mammals,and α-MSH and ACTH are endogenous nonselective agonists for MC1R.However,we found that MC1R was highly expressed in Raw 264.7 cells which were important inflammatory cells involved in the initiation of inflammatory responses.The intriguing biological results triggered the further conformation studies; it suggested that MC1R could play an important role in immunoregulation.In this study,we were to investigate the protection of MC1R on inflammation in Lipopolysaccharide (LPS) stimulated Raw 264.7 cells.The effects of the MC1R antagonist psoralen on pro-inflammatory cytokines and signaling pathways were analyzed by enzyme-linked immunosorbent assay and realtime fluorescence quantitative PCR.The results showed that the MC1R antagonist psoralen increased the level of MC 1R mRNA in Raw 264.7 cells by cumulative feedback regulation.Furthermore,it markedly inhibited the LPS-induced TNF-α,IL-6 in vitro.Our results suggest that MC1R potentially decreases the inflammation in vitro,and might be a therapeutic way to against inflammatory diseases.
MC1R Cytokines Inflammation action Psoralen Cumulative feedback regulation
Wei Chen Hai”e Qu Jianping Li Zhou Song Qinghua Huang
College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China College of Animal Science and Veterinary Medicine, Jilin Universit
国内会议
中国畜牧兽医学会动物解剖学及组织胚胎学分会第十七次学术研讨会
山西太谷
英文
42-52
2012-07-01（万方平台首次上网日期，不代表论文的发表时间）