Cloning and expression of neuron-specific enolase in the corpus luteum of dairy goats
Neuron-specific enolase (NSE) is the key molecular marker for diffuse neuroendocrine system (DNES) cells,its expression in the pregnant corpus luteum (CL) of dairy goats was studied by the immunofluorescence method and the ultra structural characteristics of luteal cells were detected by the electron microscopy to identify the existence of DNES cells in the pregnant CL of dairy goats.Besides,the coding sequence of dairy goats NSE gene was cloned and its biological information was analyzed.Results revealed that NSE immunopositive cells exhibited widespread cytoplasmic staining throughout the whole pregnant CL.In addition,these cells showed typical characteristics of DNES cells in the electron microscopy.These results suggested that many DNES cells exist in the pregnant CL of dairy goats.Meanwhile,we identified the coding sequence of dairy goats NSE (GenBank accession No.JN887466).Its nucleotide sequence homology was found to be 97.9％,89.3％,90％ and 92.6％,respectively,compared with that of Bos taurus,Rattus norvegicus,Mus musculus and Homo sapiens,while the amino acid sequence homology was 99.1％,97％,97.2％ and 98.2％ respectively.These results first showed that the functional amino acids coded by the NSE gene were highly conserved in Caprine,Bos taurus,Rattus norvegicus,Mus musculus and Homo sapiens.It was implied that the gene NSE in dairy goats had close homology to that of NSE of other species.Our findings demonstrated the possible existence of DNES cells in pregnant CL,providing new clue for further understanding of interactions between the neuroendocrine and reproductive system.Characterization of gene sequence of dairy goats NSE will enable us to synthesize interference RNA for further study on the role of NSE on the formation,function and apotosis of pregnant CL in dairy goats.
neuron-specific enolase corpus luteum diffuse neuroendocrine system immunofluorescence staining molecular cloning coding sequence
MENG Xia CHEN Shu-lin MO Hong-fei LI Fei-hu TANG Cai-yan
College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi 712100, China College of Veterinary Medicine, Northwest A & F University, Yangling, S
国内会议
中国畜牧兽医学会动物解剖学及组织胚胎学分会第十七次学术研讨会
山西太谷
英文
69-83
2012-07-01（万方平台首次上网日期，不代表论文的发表时间）