Expression of growth differentiation factor 9 (GDF9),ALK5, and claudin-ll in adult alpaca testis
Growth differentiation factor 9 (GDF9) is an oocyte-derived factor critical to folliculogenesis.Recently,in vitro data showed that GDF9 inhibited the localization of tight junction (T J) proteins,suggesting that GDF9 could potentially regulate spermatogenesis in vivo,via inhibition of Sertoli cell TJ function.The purpose of the present study was to determine the expression and localization of GDF9,its receptor,ALK5 and its latent target protein,claudin-11 (one of TJ proteins) in adult alpaca testis using Western blot and immunohistochemistry.Western blotting results demonstrated that GDF9,ALK5 and claudin-ll were expressed in the adult alpaca testis.Immunohistochemistry revealed that GDF9 was expressed stage-specifically in the cytoplasm of pachytene spermatocytes and round spermatids of adult alpaca seminiferous epithelium.Type I receptor,ALK5 was mainly localized in the cytoplasm of round spermatids and Leydig cell,to a lesser extent in the cytoplasm ofpachytene spermatocytes and Sertoli cells.Its latent target protein,claudin-11,was perpendicular or parallel to the basal lamina in the basal part of Sertoli cells.These results indicated that GDF9,as a paracrine and autocrine growth factor derived from round spermatids and pachytene spermatocytes,is involved in regulating spermatogenesis via action on germ cells or somatic cells ( i.e.Leydig cells,Sertoli cells).
Adult alpaca Testis Spermatogenesis Growth differentiation factor 9 ALK5 Claudin-11
QingYun Guo ZhenZhen Gao Li Zhao JunPing He
Institute of Animal Biotechnology, College of Animal Science and Technology, Shanxi Agricultural Uni Institute of Animal Biotechnol
国内会议
中国畜牧兽医学会动物解剖学及组织胚胎学分会第十七次学术研讨会
山西太谷
英文
102-113
2012-07-01（万方平台首次上网日期，不代表论文的发表时间）