A Rapid and Simple Method for Detection of Tobacco mosaic virus in Nicotiana tabacum by Aqueous Extract
Tobacco mosaic virus(TMV) is a widespread plant virus of the genus Tobamovirus that infects plants,especially tobacco and other members of the family Solanaceae.The infection causes mottling and discoloration on the leaves.In this study we reported the simple and rapid method for detection of TMV based on aqueous extract and agarose gel electrophoresis,and all procedures require 30 min.A small amount of tobacco leaves(～10 mg) was ground in sterile water(～100 μl),and the electrophoretic bands of about 6,000 nt in aqueous extract were detected through agarose gel electrophoresis.The Northern-Blot analysis targeting TMV CP gene showed that the electrophoretic bands was TMV genomic.However,the aqueous extract is unstable under room temperature and degraded after 5 hr extraction.Therefore,this method is more rapid,simple and economic than those methods reported previously.
Tobacco mosaic virus(TMV) detection aqueous extract
YANG Jinguang WANG Fenglong Xu Manlin SHEN Lili QIAN Yu
Key Laboratory of Tobacco Quality Control,Ministry of Agriculture,the People”s Republic of China,Qin HeZe University Resources and Environment Sci Department,Heze,Shandong 274000
国内会议
昆明
英文
387-387
2009-08-07（万方平台首次上网日期，不代表论文的发表时间）