A Real-time Reverse Transcription PCR Assay for Detection of Cucumber mosaic virus in Individual Peach Aphid(Myzus persicae)
Cucumber mosaic virus(CMV) is transmitted in a nonpersistent manner by peach aphid (Myzus persicae),leading to many crop diseases.The large number of viruliferous peach M.persicae is one of the major culprits for the rampant outbreak.A novel real-time PCR assay targeting CP gene was developed to detect CMV in individual M.persicae.The sensitivity of this method was 1000-fold higher than that of the conventional PCR.These primers,with a battery of reference virus isolates,including most isolates,showed high specificity for CMV.Additionally,this method yielded negative results on all other viruses with identical transmitting vector tested.Thus,the real time RT-PCR assay could be a valuable tool for rapid,sensitive,specific detection of CMV in M.persicae.
Cucumber mosaic virus real-time RT-PCR detection aphid(Myzus persicae)
YANG Jinguang Xu Manlin WANG Fenglong SHEN Lili QIAN Yu
Key Laboratory of Tobacco Quality Control,Ministry of Agriculture,the People”s Republic of China,Qin HeZe University Resources and Environment Sci Department,Heze,Shandong 274000
国内会议
昆明
英文
394-394
2009-08-07（万方平台首次上网日期，不代表论文的发表时间）