Cloud point extraction for speciation of Chromium(Ⅲ)and Chromium(Ⅵ) in sediment samples from Chaohu Lake by high-performance liquid chromatography
cloud point extraction(CPE) was applied as a preconcentration step for high-performance liquid chromatography(HPLC) speciation of chromium in lake sediments.Simultanous preconcentration of Cr(Ⅲ) and Cr(Ⅵ) in sediment samples was achieved by CPE with 1-(2-thiazdylazo)-2-napthol(TAN) as the chelating agent and noni onic surfactant octylphe-noxypolyethoxyethanol(Triton X-114) as the extractant.Baseline separation of the TAN chelates of Cr(Ⅲ) and Cr(Ⅵ) was realized on a RP-C18 column by using a mixture of methanol water(69∶31,v/v) solution and 4.5mM CTMAB buffered with 0.03 M NaAc HAc solution(pH 5.5) as the mobile phase at a flow rate of 0.8 mL min-1.The variables affecting the complexation and extraction steps were examined.The precision(R.S.D.) for seven replicate injections of amixture of 100(g L-1 of Cr(Ⅲ) and Cr(Ⅵ) were 1.2 and 0.9％ for the retention time,4.7 and 2.7％ for the peak area,respectively.The proposed procedure was applied to the speciation of chromium in sediment samples with satisfactory results.
Cloud point extraction Chromium speciation High-performance liquid chromatography Sediment Chaohu Lake
Wang leilei Wang jiaquan Xiao pu Zheng zhixia
School of Resources Science and Environment Engineering;Hefei University of Technology;Hefei,P.R.Chi Provincial Key Laboratory of Research on Wastewater Treatment Technology,Anhui Academy of Environmen Provincial Key Laboratory of Research on Wastewater Treatment Technology,Anhui Academy
国内会议
武汉
英文
1990-1994
2009-11-01（万方平台首次上网日期，不代表论文的发表时间）