Position Effect in hpRNA-mediated Virus Resistance Against the PVY CP Gene in Plants

Hairpin RNA (hpRNA) mediated silencing has become a powerful tool for conferring virus resistance in agriculturally important transgenic plants.However,the levels of virus resistance vary greatly,depending on the target sequence selected.In the present study,we have designed sixteen hp-RNAi constructs targeted to the Coat Protein (CP) gene of Potato virus Y (PVY),a gene that is highly conserved in sequence among different PVY isolates.The efficiency of gene silencing varied significantly among different hpRNAs targeted to different regions of gene.Hairpin constructs targeted to 5” regions of CP gene were unable to induce virus resistance,while hairpin constructs targeting 3” and middle regions induced efficient virus resistance of up to 77.8%,suggesting the existence of an extensive position effect.Northern blot analysis of the accumulation of the transcripts and the produced siRNAs showed that 50bp-length hp-RNAi constructs could be transcribed efficiently and then processed into siRNAs by the plant-self RNA silencing mechanism.
hpRNA-mediated virus resistance position effect PTGS PVY RNA silencing
WU Bin JIANG Fang SONG Yun-zhi ZHU Chang-xiang WEN Fu-jiang
State Key Laboratory of Crop Biology, Shandong Agricultural University,Tai”an, Shandong 271018, China
国内会议
郑州
英文
208-208
2009-10-24(万方平台首次上网日期,不代表论文的发表时间)