Analysis and difference of voltage-dependent anion channel mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia
Purpose:To analyze the abundance and difference of voltagedependent anion channel (VDAC) mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia.Methods:High motile and low motile spermatozoa were separated respectively from ejaculates of 36 donors and 40 patients using a discontinuous Percoll gradient centrifugation.Real-Time PCR was performed to detect mRNA abundance and difference of three VDAC subtypes between two groups with different sperm motility.Results:Real-Time PCR demonstrated that three VDAC mRNAs were present in mature spermatozoa.The VDAC2 mRNA level in ejaculated spermatozoa of patients was significantly higher than that of donors.No significant
Bianjiang Liu Peng Wang Zengjun Wang Yuejun Jia Xiaobing Niu Wei Wang Wei Zhang
Department of Urology,The First Affiliated Hospital of Nanjing Medical
国内会议
南京
英文
82-83
2011-04-15（万方平台首次上网日期，不代表论文的发表时间）