Potential contribution of matrix metalloproteinase-9 to cerebral vasospasm after experimental subarachnoid hemorrhage in rats
This study investigated the possible involvement of matrix metalloproteinase 9 ( MMP-9) in cerebral vasospasm (CVS) of subarachnoid hemorrhage (SAH) in rats.The CVS model was established by injection of fresh autologous nonheparinized arterial blood into the cisterna magna.Experiment 1 aimed to investigate the timecourse of the MMP-9 expression in the basilar artery after SAH.In experiment 2,we chose the maximum time point of vasospasm( Day 3 ) and assessed the effect of SB-3CT( a selective MMP-9 inhibitor) on regulation of cerebral vasospasm.Cross-sectional area of basilar artery was measured by H&E staining and the MMP-9 expression was assessed by immunohistochemistry analysis.The elevated expression of MMP-9 was detected in the basilar artery after SAH and peaked on day 3.After intracisternal administration of SB-3CT,the vasospasm was markedly attenuated after blood injection on day 3.Our results suggest that MMP-9 is increasingly expressed in a parallel time course to the development of cerebral vasospasm in a rat experimental model of SAH and administration of the specific MMP-9 inhibitor could prevent or reduce cerebral vasospasm caused by SAH.
Zhong Wang Gang Chen SAH Research Team
Department of Neurosurgery, The First Affiliated Hospital of Soochow University
国内会议
北京
英文
58-58
2012-08-10（万方平台首次上网日期，不代表论文的发表时间）