Expression of Nemo-like kinase (NLK) in the brain in a rat experimental subarachnoid haemorrhage model
The present study aimed to investigate the expression of the Nemo-like kinase (NLK) in the brain after experimental subarachnoid haemorrhage (SAH) in rats.A total of 36 rats were randomly divided into six groups:control group; day 1,day 3,day 5,day 7 and day 14 groups.Day 1,day 3,day 5,day 7 and day 14 groups were all SAH groups in which the rats were killed on day 1,3,5,7 and 14,respectively.In SAH groups,autologous arterial blood was injected into cisterna magna once on day 0.Immunostaining and immunoblotting experiments were performed to detect the expression of NLK protein.Real-time polymerase chain reaction (PCR) was used to analyze the presence and quantity of NLK mRNA.The expressions of NLK protein and mRNA were decreased remarkably in SAH groups compared with the control group. The immunohistochemical staining demonstrated expression of NLK was present mainly in the neurons in the brain.Our results indicate that NLK expression is down-regulated in the brain after experimental SAH.
Gang Chen Zhong Wang SAH Research Team
Department of Neurosurgery, The First Affiliated Hospital of Soochow University
国内会议
北京
英文
59-59
2012-08-10（万方平台首次上网日期，不代表论文的发表时间）