Molecular characterization of a novel 14-3-3 protein gene (Hb1 4-3-3c) from Hevea brasiliensis
The cDNA encoding a 14-3-3 protein, designated as Hb14-3-3c, was isolated from Hevea brasiliensis. Hb14-3-3c was 1269 bp long containing a 795 bp open reading frame encoding a putative protein of 264 amino acids, flanked by a 146 bp 5”UTR and a 328 bp 3”UTR. The predicted molecular mass of Hb14-3-3c is 29.67 kDa, with a isoelectric point of 4.52 and the deduced protein showed high similarity to the 14-3-3 protein from other plant species. Expression analysis revealed more significant accumulation of Hb14-3-3c transcripts in latex than in leaves, buds and flowers. The transcription of Hb14-3-3c in latex was induced by jasmonate and ethephon. Overproduction of recombinant Hb14-3-3c protein gave the Escherichia coli cells more tolerance on Co2+, Cu2+ and Zn2+. Through yeast two-hybrid screening, 11 interaction partners of the Hb14-3-3c, which are involved in rubber biosynthesis, stress-related responses, defence etc, were identified in rubber tree latex. Taking these data together, it is proposed that the Hb14-3-3c may participate in regulation of rubber biosynthesis. Thus, the results of this study provide novel insights into the 14-3-3 signaling related to rubber biosynthesis, stress-related responses in rubber tree.
14-3-3 protein Hevea brasiliensis jasmonate heavy metal ions interaction partner
Zi-Ping Yang Hui-Liang Li Dong Guo Wei-Min Tian Shi-Qing Peng
Key laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Biosci Key Laboratory of Rubber Biology, Ministry of Agriculture, Institute of Rubber, Chinese Academy of T
国内会议
银川
英文
1-4
2012-10-15（万方平台首次上网日期，不代表论文的发表时间）